Journal: Cancers
Article Title: Crosstalk Between Omental Adipose-Derived Stem Cells and Gastric Cancer Cells Regulates Cancer Stemness and Chemotherapy Resistance
doi: 10.3390/cancers16244275
Figure Lengend Snippet: ( A ) Human cytokine array detecting multiple cytokines, chemokines, growth factors in a cell culture medium. ASCs were cultured alone or co-cultured indirectly with MKN45 cells in serum-free media for 48 h. The levels of various factors in culture media were measured using a proteome profiler array and normalized to positive control according to the manufacturer’s protocol. Data are expressed as fold inductions from duplicate experiments. ( B ) IL-6 concentrations secreted from ASCs were examined using ELISA. A conditioned medium from all GC cell lines significantly promoted IL-6 secretion from ASCs. Data represent the mean ± SD of triplicate wells for three independent experiments (** p < 0.01).
Article Snippet: To determine IL-6 concentrations in ASC culture media, we employed a commercially available Human IL-6 Quantikine ELISA kit following the protocol provided by the manufacturer (R&D Systems).
Techniques: Cell Culture, Positive Control, Enzyme-linked Immunosorbent Assay